LDH continuous mode assay A total of 130 µl of serially diluted PKA combined with 40 µM kemptide substrate were added to wells of a white 96well optical plate (Costar) A total of µl 10X LDH assay reagent (3 mM NADH, 1 mM PEP, 30 U/ml LDH Sigma, U/ml pyruvate kinase in 100 mM Hepes, mM MgCl 2, µg/ml bovine serum albumin, 01
